Stephen O'Brien, the UN humanitarian affairs chief, called for an inquiry into the attack on the Kamouna camp in the northern Idlib province.
Reports say the strike on the rebel-held area was by Syrian or Russian planes but this has not been confirmed.
Thursday's attack came a day after the extension of a truce was confirmed.
The Syrian military and non-jihadist rebel forces had agreed to a temporary truce around the city of Aleppo, following pressure from the US and Russia.
But the UK-based monitoring group, Syrian Observatory for Human Rights (SOHR), reported an upsurge in fighting between Syrian forces and al-Qaeda-affiliated jihadists close to Aleppo, killing more than 70 fighters from both sides.
The insurgents, including the al-Nusra Front, are reported to have seized the strategically important village of Khan Tuman back from government forces, which had captured it in December.
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